(1 ) Briefly press the “Power” button to allow the system to warm up. Wait for about three minutes. (2)Check that the shutter located immediately after of the output slit of the Tsunami is in the “closed” position, so as to prevent the laser beam from reaching the optical table. (3) Once the display screen announces that the optimal temperature has been reached, press and hold down the “Power” button. The “Laser Emission” indicator located beside the “Power” button will start to sparkle. Keep pressing down the “Power” button until the “Laser Emission” indicator ceases to sparkle and shines with a stable, bright red colour. (4) Increase the current of the laser until you reach a value of 20%. Wait for three minutes. (5) Increase the current of the laser by 10%. Wait three more minutes. (6) Repeat the previous step until the Tsunami reaches a current of 90%. Then increase the current by 1% (i.e. to 91%) and wait for another three minutes. (7) Press the “Setup” button and then scroll down and change from “Current” mode to “Power” mode. (8) Increase the laser power to 6.0-6.5 W. (9) Place a power meter in the optical path. Place it between the output slit of the Tsunami and the BBO crystal, so as to measure the power of the fundamental laser beam. (10) Check that you are using protection googles. If you are, open the shutter mentioned in step 2. (11) Adjust the vertical output couplers (M1 and M10) located at the right and left ends of the Tsunami until you maximize the laser power. Then adjust the horizontal output couplers located at the right and left ends of the Tsunami until you maximize the laser power. Come back to adjusting the vertical output couplers. Repeat the process iteratively until you reach an overall maximum laser power. NOTE: never adjust the horizontal AND vertical output couplers at the same time. (12) Modelock the laser by adjusting the slit wavelength selector and the prism dispersion compensation control (both of these knobs are located at the centre of the laser box). If the laser is properly modelocked, the “Pulsing” indicator will turn from red to green. Sometimes, a slight tap on the top of the laser box may prove helpful when it comes to modelocking the laser. (13) Select the desired wavelength by adjusting the wavelength selector and then broaden the laser pulse as much as possible by adjusting the prism dispersion compensation control. The FWHM of the fundamental laser beam should have a value of at least 13 nm. (14) Place the power meter between the BBO crystal and the sample holder, so as to measure the power of the frequency-doubled laser beam. (15) Adjust the position of the BBO crystal to maximize the power of the frequency-doubled laser beam. (16) Remove the power meter from the optical path to allow the frequency-doubled laser beam to reach the sample. (17) Start a “Live Acquisition” in the streak camera using the “Focus” mode. Be careful not to expose the streak camera to an excessive amount of light. Use a small streak camera slit opening and an appropriate optical filter. (18) Adjust the position of the doublet prism placed immediately before the streak camera, so as to maximize the intensity of the signal read by the streak camera. (19) Adjust the position of the sample holder to maximize the intensity of the signal read by the streak camera. (20) Repeat steps 18 and 19 iteratively until the signal read by the streak camera reaches an overall maximum. (21) Set the streak camera into “Operation” mode. (22) Adjust the optical density wheel located in front of the trigger of the streak camera. This will modify the position of the signal on the display of streak camera software. Adjust the position of the wheel in order to make the signal as temporally narrow as possible. The rise time of the emission decay should be 20 ps for time resolution 4 (TR4). If you are observing the frequency doubled laser beam instead of the emission of a sample, the FWHM of the laser beam should have a value of about 20 ps, provided that the camera is in TR4.